Difference between revisions of "Part:BBa K2100036"
 
Line 3: Line 3:
 
<partinfo>BBa_K2100036 short</partinfo>
 
<partinfo>BBa_K2100036 short</partinfo>
  
−
This part is the result of a gateway combining a tet response element promoter (TRE) and the repressor BM3R1. The repressor can bind to sites on the BM3R1 promoter.  
+
This construct is an expression vector containing the TRE promoter upstream of the BM3R1 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the BM3R1 repressor. BM3R1 represses the DNA sequences found on the pBM3R1 promoter with the help of the corepressor VP16-Gal4.  
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 05:56, 19 October 2016


pEXPR TRE-BM3R1

This construct is an expression vector containing the TRE promoter upstream of the BM3R1 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the BM3R1 repressor. BM3R1 represses the DNA sequences found on the pBM3R1 promoter with the help of the corepressor VP16-Gal4.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 580
    Illegal XbaI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 580
    Illegal NheI site found at 453
    Illegal NotI site found at 342
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 580
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 580
    Illegal XbaI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 580
    Illegal XbaI site found at 30
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 446
    Illegal BsaI site found at 573