Difference between revisions of "Part:BBa K1161003"

 
(Added reference to codon-optimized part as contribution)
Line 4: Line 4:
  
 
Violacein C (Vio)C is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.
 
Violacein C (Vio)C is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.
 +
 +
<h2>Contributions</h2>
 +
<p><b>Summary:</b> Our team has codon optimized this part for use in S. cerevisiae.  The new part, along with its further documentation, can be found at https://parts.igem.org/Part:BBa_K2165000
 +
as part K2165000</p>
 +
<p><b>Group:</b> Washington iGEM team 2016.</p>
 +
<p><b>Authors:</b> Cameron Roots, Natalie Johntson, Roya Amini-Naieni</p>
 +
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
Line 11: Line 19:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1161003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1161003 SequenceAndFeatures</partinfo>
 +
<!-- Part Contributions
 +
  
  

Revision as of 05:53, 19 October 2016


Violacein C

Violacein C (Vio)C is the first of 5 protein coding sequences contained in the Violacein operon originally derived from the organism, Chromobacterium violaceum. The entire operon was submitted to the registry by the Cambridge team in 2009 as K274002. Together these five proteins form a metabolic pathway that produces the violet pigment violacein when expressed in E. coli. Like its other four partners we have appended the coding sequence with the ribosome binding site BBa_B003 so that operons that vary in gene order can be created by standard assembly means. Here we use these parts to show gene order affects pigment colour.

Contributions

Summary: Our team has codon optimized this part for use in S. cerevisiae. The new part, along with its further documentation, can be found at https://parts.igem.org/Part:BBa_K2165000 as part K2165000

Group: Washington iGEM team 2016.

Authors: Cameron Roots, Natalie Johntson, Roya Amini-Naieni


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1312
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]