Difference between revisions of "Part:BBa K2130002"

 
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K2130002 short</partinfo>
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<partinfo>BBa_K2130002 Mutant Cas9 459</partinfo>
  
 
This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.
 
This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.

Revision as of 04:43, 19 October 2016


BBa_K2130002 Mutant Cas9 459 Not understood

This part is the CDS of Cas9 from Streptococcus pyogenes, which contains single amino acids substitution created via random mutation and selected via evolution in the lab.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3643
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
    Illegal BglII site found at 1647
    Illegal BglII site found at 1736
    Illegal BglII site found at 2381
    Illegal BamHI site found at 4105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]