Difference between revisions of "Part:BBa K2100030"

Revision as of 03:22, 19 October 2016

pEXPR pPRE3:eYFP The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.

When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain. The DBD can then bind to the PREs on the promoter, recruiting transcriptional machinery and activating transcription.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 211
Illegal PstI site found at 466
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 211
Illegal PstI site found at 466
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 211
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 211
Illegal PstI site found at 466
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 211
Illegal PstI site found at 466
Illegal AgeI site found at 249
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 7
Illegal BsaI.rc site found at 225

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K2100030 short</partinfo>
+The entry vector contains the PREx3 promoter, consisting of 3 progesterone responsive elements of the sequence 5'-G/A G G/T AC A/G TGGTGTTCT-3' upstream of a minimal CMV promoter. The PREs are interspaced according to the spacing on the TRE promoter.
-description here.
+When progesterone diffuses through the cell membrane of an endometrial cell, it binds to the ligand binding domain on PgR-beta (Progesterone Receptor beta) and exposes its DNA binding domain. The DBD can then bind to the PREs on the promoter, recruiting transcriptional machinery and activating transcription.
 <!-- Add more about the biology of this part here