Difference between revisions of "Part:BBa K2066029:Design"

 
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===Source===
 
===Source===
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Codons were optimized for E. Coli expression and non compatible restriction sites were removed.
 
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===References===
 
===References===

Revision as of 03:03, 19 October 2016


pBad - TetR/mCherry Fusion


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1245
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1019
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1001


Design Notes

Linker and mCherry taken from Synthetic Analog Computation in Living Cells (Daniel et al.). TetR from NCBI NC_011812.1



Source

References