Difference between revisions of "Part:BBa K2040120:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | One illegal cutsite (EcoRI) were subsequently removed via site-directed mutagenesis. | |
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===Source=== | ===Source=== | ||
Latest revision as of 02:59, 19 October 2016
KillerRed + TtrpC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 151
Illegal BsaI.rc site found at 442
Design Notes
One illegal cutsite (EcoRI) were subsequently removed via site-directed mutagenesis.
Source
KillerRed was engineered from anm2CP.1 TtrpC originated from Aspergillus nidulans was isolated from pBARGPE1.