Difference between revisions of "Part:BBa K2040121"

Revision as of 02:36, 19 October 2016

PgpdA + KillerRed + TtrpC

A KillerRed expression cassette was created with a fungal promoter PgpdA and a fungal terminator TtrpC.

Usage and Biology

KillerRed,always used for precise inactivation of selected proteins in chromophore-assisted light inactivation (CALI) technique and for the light-induced cell killing, is rarely used to express in fungal chassis.

NYMU 2016 construct this new device, for fungal expression, to test wheather KillerRed protein can be used in entomopathogenic fungi Metarhizium anisopliae, our chassis organism.

We demonstrated the functionality of this part via transforming this expression cassette into M.anisopliae. The transformants were under a fluorescence microscope(Magnification: 400X):

• Wildtype:

• KillerRed transformants:

This new device was successfully expressed in M.anisopliae.

Sequence and Features