Difference between revisions of "Part:BBa K2150113"

 
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<h2>toxin ParE2 induced by aTc</h2>
  
__NOTOC__
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===Usage and Biology===
<partinfo>BBa_K2150113 short</partinfo>
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a
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This part is used to build a kill-switch.
  
<!-- Add more about the biology of this part here
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===Characterization===
===Usage and Biology===
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<h4>Growth Curve</h4>
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We measured the growth curve of bacteria expressing toxin ParE2.
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[[file:T--UCAS--6249t-atc.png|500px]]
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The growth curve of wild type E. coli harboring empty vector is shown below.
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[[file:T--UCAS--RGP_Ptet.png|500px]]
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This construction is also used to identify the interaction between toxins and antitoxins.
  
 
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Revision as of 02:07, 19 October 2016

toxin ParE2 induced by aTc

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin ParE2.

T--UCAS--6249t-atc.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptet.png

This construction is also used to identify the interaction between toxins and antitoxins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 455
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]