Difference between revisions of "Part:BBa K2150110"

Revision as of 01:49, 19 October 2016

This part is used to build a kill-switch.

We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.

The growth curve of wild type E. coli harboring empty vector is shown below.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 1611
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1857
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 1611
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 1611
Illegal NgoMIV site found at 1788
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
-__NOTOC__
+<h2>toxin N-acetyltransferase GCN5 induced by IPTG</h2>
-<partinfo>BBa_K2150110 short</partinfo>
-This composite part consists of LacI under constitutive promoter, and toxin gene of toxin N-acetyltransferase GCN5 (In our project, ID: 1204) under pTac prompter.
-Directly upstream of coding region of toxin gene is RiboJ, an insulator buffers gene expression from its cellular context. RIboJ is a ribozyme which effetely cleaves 5'-UTR of mRNAs.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-We used this device to test the toxicity of toxin protein. Different amount of IPTG is added, and the growth curve of bacteria is measured using 96-well microplate spectrophotometer.
+This part is used to build a kill-switch.
+===Characterization===
+<h4>Growth Curve</h4>
+We measured the growth curve of bacteria expressing toxin N-acetyltransferase GCN5.
+[[file:T--UCAS--1204t-IPTG.png|500px]]
+The growth curve of wild type E. coli harboring empty vector is shown below.
+[[file:T--UCAS--RGP_Ptac.png|500px]]
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>