Difference between revisions of "Part:BBa K2150119"
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===Characterization===
 
===Characterization===
 
<h4>Growth Curve</h4>
 
<h4>Growth Curve</h4>
−
We measured the growth curve of bacteria expressing toxin ParE2
+
We measured the growth curve of bacteria expressing toxin ParE2.
 +
 
 
[[file:T--UCAS--6249t-IPTG.png|500px]]
 
[[file:T--UCAS--6249t-IPTG.png|500px]]
 +
 +
The growth curve of wild type E. coli harboring empty vector is shown below.
 +
 +
[[file:T--UCAS--RGP_Ptac.png|500px]]
  
 
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Revision as of 01:38, 19 October 2016

toxin ParE2 induced by IPTG

Usage and Biology

This part is used to build a kill-switch.

Characterization

Growth Curve

We measured the growth curve of bacteria expressing toxin ParE2.

T--UCAS--6249t-IPTG.png

The growth curve of wild type E. coli harboring empty vector is shown below.

T--UCAS--RGP Ptac.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1600
  • 1000
    COMPATIBLE WITH RFC[1000]