Difference between revisions of "Part:BBa K2086000:Design"
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===Source=== | ===Source=== | ||
− | + | The napDABC gene sequence contains parts derived from the genomic DNA of <i>E. coli</i> (MG1655). Certain sequences were changed and ordered from IDT in order to eliminate illegal restriction sites. | |
===References=== | ===References=== |
Revision as of 01:02, 19 October 2016
napDABC (Periplasmic Nitrate Reductase)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1991
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 479
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 324
Illegal SapI.rc site found at 238
Illegal SapI.rc site found at 1734
Design Notes
The part had an illegal restriction site in the NapA gene. The NapDA genes and NapBC genes aren't normally adjacent to each other and so were assembled using HiFi assembly.
Source
The napDABC gene sequence contains parts derived from the genomic DNA of E. coli (MG1655). Certain sequences were changed and ordered from IDT in order to eliminate illegal restriction sites.