Difference between revisions of "Part:BBa K2065005:Experience"
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===Applications of BBa_K2065005===
 
===Applications of BBa_K2065005===
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This construct was successfully created at DNA level and after this the protein was expressed. After this we did several assays with the protein, which functioned as scaffold for other proteins.  
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==iGEM TU Eindhoven 2016==
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iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This system was linked to CT52 (CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007") These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki.
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[[File:T--TU-Eindhoven--NanolUcreadoutbb.png]]
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''Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52''
  
  

Revision as of 00:02, 19 October 2016

Applications of BBa_K2065005

iGEM TU Eindhoven 2016

iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This system was linked to CT52 (CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007") These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki.

T--TU-Eindhoven--NanolUcreadoutbb.png
Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52


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