Difference between revisions of "Part:BBa K2065002:Experience"
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==iGEM TU Eindhoven 2016== | ==iGEM TU Eindhoven 2016== | ||
iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This was accomplished by linking CT52, the protein which has affinity to the T14-3-3 and complementary mutant form to parts of this reporter system | iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This was accomplished by linking CT52, the protein which has affinity to the T14-3-3 and complementary mutant form to parts of this reporter system | ||
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| + | iGEM TU Eindhoven used this part in combination with the NanoBiT system linked to CT52(CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007" These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki. | ||
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| + | [[File:T--TU-Eindhoven--NanolUcreadoutbb.png]] | ||
| + | <br> | ||
| + | ''Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52'' | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 00:00, 19 October 2016
Applications of BBa_K2065002
iGEM TU Eindhoven 2016
iGEM TU Eindhoven created this scaffold protein by computational design and checked its functioning and orthogonality using the NanoBiT reporter system. This was accomplished by linking CT52, the protein which has affinity to the T14-3-3 and complementary mutant form to parts of this reporter system
iGEM TU Eindhoven used this part in combination with the NanoBiT system linked to CT52(CT52-SmallBiT"https://parts.igem.org/Part:BBa_K2065000" and CT52-LargeBiT"https://parts.igem.org/Part:BBa_K2065007" These two proteins could dimerize on our heterodimeric T-14-3-3 scaffold proteins with mutation which were designed by our team. When dimerized the functional luciferase protein was formed. This protein showed luminescence at 460 nm. Thus this system was used as a read out method for T14-3-3 heterodimers. This system is schemtically visualised in figure 1. For more detailed information about the application of CT52-SmallBiT visit our wiki.
Figure 1: Measurement system for T14-3-3 heterodimers using the NanoBiT system linked to CT52
User Reviews
UNIQ522e50d7fe8cd46f-partinfo-00000000-QINU UNIQ522e50d7fe8cd46f-partinfo-00000001-QINU