Difference between revisions of "Part:BBa K2149017:Design"
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The gene of the aldehyde decarbonylase was taken from Synechococcus elongatus, a unicellular cyanobacterium and add after the genes of operon Lux. | The gene of the aldehyde decarbonylase was taken from Synechococcus elongatus, a unicellular cyanobacterium and add after the genes of operon Lux. | ||
| − | + | HOWARD, T. P. et al. Synthesis of customized petroleum-replica fuel molecules by targeted modi fi cation of free fatty acid pools in Escherichia coli. PNAS, p. 1–6, 2013. | |
Revision as of 23:54, 18 October 2016
Operon Lux C,E and D with an aldehyde decarbonylase
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2443
Illegal NheI site found at 3827 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3215
Illegal BamHI site found at 552
Illegal BamHI site found at 2382
Illegal BamHI site found at 3608 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 466
Illegal AgeI site found at 2185
Illegal AgeI site found at 2400 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3649
Illegal SapI.rc site found at 2020
Design Notes
This construction has a RBS before each gene (Lux C, Lux E and Lux D) and a UTR sequence before the gene that encodes an aldehyde decarbonylase.
Source
The sequences of operon Lux C, E and D was taken from Photorhabdus luminescens as describe by the work of Dr. Howard and collaborators called "Synthesis of customized petroleum-replica fuel molecules by targeted modification of free fatty acid pools in Escherichia coli". The gene of the aldehyde decarbonylase was taken from Synechococcus elongatus, a unicellular cyanobacterium and add after the genes of operon Lux.
HOWARD, T. P. et al. Synthesis of customized petroleum-replica fuel molecules by targeted modi fi cation of free fatty acid pools in Escherichia coli. PNAS, p. 1–6, 2013.