Difference between revisions of "Part:BBa K2065001:Experience"
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''Figure 1: Schematic representaton of the BRET system with tetrameric T14-3-3 scaffold protein and the NanoBiT system linked to CT52'' | ''Figure 1: Schematic representaton of the BRET system with tetrameric T14-3-3 scaffold protein and the NanoBiT system linked to CT52'' | ||
Revision as of 23:51, 18 October 2016
Applications of BBa_K2065000
iGEM TU Eindhoven 2016
We succeeded in creating this onstruct. Because the construct which on which mNeonGreen was used did finish just too late to do in vitro assays with, namely a BRET assay. For this BRET assay, the CT52-mNeongreen as well as CT52-LargeBit and CT52-SmallBit were assembled on the T14-3-3 scaffold protein. CT52-mNeonGreen will be the acceptor that changes the wavelength of the light emitted by NanoLuc to a wavelength of 517 nm. This system is visible in figure 1.
Figure 1: Schematic representaton of the BRET system with tetrameric T14-3-3 scaffold protein and the NanoBiT system linked to CT52