Difference between revisions of "Part:BBa K1930006"

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===Usage and Biology===
 
===Usage and Biology===
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<em>E.coli</em> appears green, when grown with this BioBrick.
  
 
<html> <a> <img src="https://static.igem.org/mediawiki/parts/b/b0/T--Groningen--sfgfp-plate.jpeg" height=400"/></a></html>
 
<html> <a> <img src="https://static.igem.org/mediawiki/parts/b/b0/T--Groningen--sfgfp-plate.jpeg" height=400"/></a></html>
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Figure 1. sfGFP(Sp) in <em>E.coli</em> Top 10.
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When the sfGFP is integrated in the genome of <em>B. subtilis</em> 168 the colonies do not appear green by eye but under the microscope they are very well green.
  
 
<html> <a> <img src="https://static.igem.org/mediawiki/parts/6/61/T--Groningen--sfgfp-microscopy.jpeg" height=400"/></a></html>
 
<html> <a> <img src="https://static.igem.org/mediawiki/parts/6/61/T--Groningen--sfgfp-microscopy.jpeg" height=400"/></a></html>
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Figure 2. sfGFP(Sp) in <em>B. subtilis</em> 168
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Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.
 
Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.
  

Revision as of 23:38, 18 October 2016


sfGFP(Sp)

sfGFP(Sp) is a reporter gene originally optimized for Streptococcus pneumoniae.

It has been shown when expressed in B. subtilis that the signal is even brighter than the one from sfGFP(Bs). [http://aem.asm.org/content/79/20/6481.full]


Usage and Biology

E.coli appears green, when grown with this BioBrick.

Figure 1. sfGFP(Sp) in E.coli Top 10.

When the sfGFP is integrated in the genome of B. subtilis 168 the colonies do not appear green by eye but under the microscope they are very well green.

Figure 2. sfGFP(Sp) in B. subtilis 168

Images were taken with an Olympus IX71 microscope (Personal DV, Applied Precision; assembled by Imsol, Preston, United Kingdom) using CoolSNAP HQ2 camera (Princeton Instruments, Trenton, NJ, USA) with a 100× phase-contrast objective. Fluorescence filter sets (excitation, 450 to 490 nm; emission, 500 to 550 nm) used to visualize GFP were from Chroma Technology Corporation (Bellows Falls, VT, USA). The exposure times were 0.2 s with 32% excitation xenon light (300 W) for B. subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]