Difference between revisions of "Part:BBa K1941003:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The overall structure of the scRNA was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting CYC1 in the c6 region of the promoter, as described in | |
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===Source=== | ===Source=== | ||
Revision as of 23:09, 18 October 2016
scCyc1_PP7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 180
Illegal NgoMIV site found at 209 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The overall structure of the scRNA was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting CYC1 in the c6 region of the promoter, as described in
Source
some source!