Difference between revisions of "Part:BBa K1941003"
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<partinfo>BBa_K1941003 short</partinfo> | <partinfo>BBa_K1941003 short</partinfo> | ||
| − | Scaffold RNA that targets the region c6 of CYC1 promoter and | + | Scaffold RNA with PP7 hairpin that targets the region c6 of CYC1 promoter |
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| + | "Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas", F. Farzadfard et al., ASC Synth Biol. 2013 | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | |||
| + | A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpin is PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1. | ||
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Revision as of 23:07, 18 October 2016
scCyc1_PP7
Scaffold RNA with PP7 hairpin that targets the region c6 of CYC1 promoter
"Tunable and Multifunctional Eukaryotic Transcription Factors Based on CRISPR/Cas", F. Farzadfard et al., ASC Synth Biol. 2013
Usage and Biology
A scaffold RNA is a guide RNA (gRNA), supplemented with one or more additional hairpins that can recruit different RNA binding modules. In this case, the hairpin is PP7, a well-characterized viral RNA sequence which is recognized by PCP RNA binding module. This allows the recruitment of fusion proteins, like BBa_K1941000, to act on the level of expression of the promoter CYC1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 180
Illegal NgoMIV site found at 209 - 1000COMPATIBLE WITH RFC[1000]