Difference between revisions of "Part:BBa K1998013"

Revision as of 22:48, 18 October 2016

MgPPIX to Chlorophyll a plasmid

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 912
Illegal BglII site found at 2365
Illegal BglII site found at 5647
Illegal BglII site found at 6136
Illegal BamHI site found at 316
Illegal BamHI site found at 3128
Illegal BamHI site found at 5416
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1904
Illegal AgeI site found at 1955
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 478
Illegal BsaI.rc site found at 4018

Overview

This composite part makes up one of our operons in the chlorophyll biosynthesis pathway. It is composed of YCF54, ChlM, POR, DVR1, ChlP and ChlG. These genes are Mg-protoporphyrin IX monomethyl ester (oxidative) cyclase (YCF54) and Magnesium-protoporphyrin O-methyltransferase (ChlM), Light-dependent protochlorophyllidereductase (POR), 3,8-divinyl, Geranylgeranyl reductase(ChlP), protochlorophyllidea 8-vinyl reductase (DVR1) and Chlorophyll synthetase (ChlG).
These genes are part of the chlorophyll biosynthetic pathway and are involved in converting Mg-protoporphyrin IX to chlorophyll

Biology & Literature

Protein information

YCF54
Mass: 17.08kDa
Sequence: MAPAAASADKATAAEYYALVCNAEWFFMDPQNESVAEQLREKVRFFKEQNKERDFFIVPNPKWLDAKFPEQAKQVKRPCVALVSTDKMWITFMKLRLDRVLKIDLKSM PASEVLAAGEALPDFKPDGKWTAPYARYTPGWWNVFLPNH

ChlM
Mass: 30.45kDa Sequence: MASEIAQTADVGSLTFAVGGVGAVVGLGALLVATDHQKRRSEQMKSFDGDEKEAVKDYFNTAGFERWRKIYGETDEVNKVQLDIRTGHAQTVDKVLRWVDEEGSVQGIT VADCGCGTGSLAIQLALRGAAVSASDISAAMASEAEQRYQQAVAAGQGKAPKVAPKFEALDLESVKGKYDTVTCLDVMIHYPQDKVDAMITHLAGLSDRRLIISFAPKTL SYSILKRIGELFPGPSKATRAYLHREEDVEAALKRAGFKVTKREMTATSFYFSRLLEAIRE

POR
Mass: 41.87kDa
Sequence:
MVVCAATATAPSPSLADKFKPNAIARVPATQQKQTAIITGASSGLGLNAAKALAATGEWHVVMACRDFLKAEQAAKKVGMPAGSYSILHLDLSSLESVRQFVQNFKASGR RLDALVCNAAVYLPTAKEPRFTADGFELSVGTNHLGHFLLTNLLLDDLKNAPNKQPRCIIVGSITGNTNTLAGNVPPKANLGDLSGLAAGVPAANPMMDGQEFNGAKAYK DSKVACMMTV RQMHQRFHDATGITFASLYPGCIAETGLFREHVPLFKTLFPPFQKYITKGYVSEEEAGRRLAAVISDPKLNKSGAYWSWSSTTGSFDNK

DVR1
Mass: 37kDa
Sequence:
MAMAASRQAVRVAAAVDADYRKREPKDVRVLVVGPTGYIGKFVVKELVSRGYNVVAFARENAGIKGKMGREDIVKEFHGAEVRFGSVLDPASLRDVAFKDPVDVVVSCLA SRTGGKKDSWLIDYTATKNSLDVARASGAKHFVLLSAICVQKPLLEFQKAKLQFESDLQAAGDITYSIVRPTAFFKSIAGQIDIVKKGNPYVMFGDGNLAACKPISEADLASF IADCVTEQNKVNKVLPIGGPSKAFTAKQQADLLFNITGLPPKYFPVPVALMDGMIGLFDSLAKLFPQLEDSAEFARIGKYYATESMLVYDEARGVYRKTKRLVTARTRWKTS SLVQ

ChlP
Mass: 47kDa
Sequence:
MVIGGGPSGACAAETLAKGGVETFLLERKLDNCKPCGGAIPLCMVEEFDLPMEIIDRRVTKMKMISPSNREVDVGKTLSETEWIGMCRREVFDDYLRNRAQKLGANIVNGL FMRSEQQSAEGPFTIHYNSYEDGSKMGKPATLEVDMIIGADGANSRIAKEIDAGEYDYAIAFQERIRIPDDKMKYYENLAEMYVGDDVSPDFYGWVFPKYDHVAVGTGTVVN KTAIKQYQQATRDRSKVKTEGGKIIRVEAHPIPEHPRPRRCKGRVALVGDAAGYVTKCSGEGIYFAAKSGRMAAEAIVEGSANGTKMCGEDAIRVYLDKWDRKYWTTYKVLD ILQKVFYRSNPAREAFVELCEDSYVQKMTFDSYLYKTVVPGNPLDDVKLLVRTVSSILRSNALRSVNSKSVNVSFGSKANEERVM AA

ChlG
Mass: 36.84kDa
Sequence:
MNQQATEEKSDTNSAARQMLGMKGAALETDIWKIRVQLTKPVTWIPLIWGVACGAAASGHYQWNNPTQIAQLLTCMMMSGPFLTGYTQTINDWYDREIDAINEPYRPIPS GRISERDVIVQIWVLLLGGIGLAYTLDQWAGHTTPVMLQLTIFGSFISYIYSAPPLKLKQSGWAGNYALGSSYIALPWWAGQALFGTLTLDVMALTIAYSLAGLGIAIVNDFKSI EGDRQ MGLQSLPVAFGVDTAKWICVSTIDVTQLGVAAYLAWGLHEELYGAVLLALILPQIYFQYKYFLPDPIANDVKYQASAQPFLVFGLLTAGLACGHHVNAVAA AASAAGAL

References

[1]

@@ Line 28: / Line 28: @@
 YCF54<br>
 Mass: 17.08kDa <br>
-Sequence: MAPAAASADKATAAEYYALVCNAEWFFMDPQNESVAEQLREKVRFFKEQN KERDFFIVPNPKWLDAKFPEQAKQVKRPCVALVSTDKMWITFMKLRLDRV LKIDLKSMPASEVLAAGEALPDFKPDGKWTAPYARYTPGWWNVFLPNH <br>
+Sequence:
+MAPAAASADKATAAEYYALVCNAEWFFMDPQNESVAEQLREKVRFFKEQNKERDFFIVPNPKWLDAKFPEQAKQVKRPCVALVSTDKMWITFMKLRLDRVLKIDLKSM
+PASEVLAAGEALPDFKPDGKWTAPYARYTPGWWNVFLPNH
 <br><br>
 ChlM<br>
 Mass: 30.45kDa
-Sequence: MASEIAQTADVGSLTFAVGGVGAVVGLGALLVATDHQKRRSEQMKSFDGD EKEAVKDYFNTAGFERWRKIYGETDEVNKVQLDIRTGHAQTVDKVLRWVD EEGSVQGITVADCGCGTGSLAIQLALRGAAVSASDISAAMASEAEQRYQQ AVAAGQGKAPKVAPKFEALDLESVKGKYDTVTCLDVMIHYPQDKVDAMIT HLAGLSDRRLIISFAPKTLSYSILKRIGELFPGPSKATRAYLHREEDVEAALK
+Sequence:
-RAGFKVTKREMTATSFYFSRLLEAIRE
+MASEIAQTADVGSLTFAVGGVGAVVGLGALLVATDHQKRRSEQMKSFDGDEKEAVKDYFNTAGFERWRKIYGETDEVNKVQLDIRTGHAQTVDKVLRWVDEEGSVQGIT
+VADCGCGTGSLAIQLALRGAAVSASDISAAMASEAEQRYQQAVAAGQGKAPKVAPKFEALDLESVKGKYDTVTCLDVMIHYPQDKVDAMITHLAGLSDRRLIISFAPKTL
+SYSILKRIGELFPGPSKATRAYLHREEDVEAALKRAGFKVTKREMTATSFYFSRLLEAIRE
 <br><br>
-POR
+POR<br>
-Mass: 41.87kDa
+Mass: 41.87kDa<br>
-Sequence:
+Sequence:<br>
-MVVCAATATA PSPSLADKFK PNAIARVPAT QQKQTAIITG ASSGLGLNAA KALAATGEWH
+MVVCAATATAPSPSLADKFKPNAIARVPATQQKQTAIITGASSGLGLNAAKALAATGEWHVVMACRDFLKAEQAAKKVGMPAGSYSILHLDLSSLESVRQFVQNFKASGR
-VVMACRDFLK AEQAAKKVGM PAGSYSILHL DLSSLESVRQ FVQNFKASGR RLDALVCNAA
+RLDALVCNAAVYLPTAKEPRFTADGFELSVGTNHLGHFLLTNLLLDDLKNAPNKQPRCIIVGSITGNTNTLAGNVPPKANLGDLSGLAAGVPAANPMMDGQEFNGAKAYK
-VYLPTAKEPR FTADGFELSV GTNHLGHFLL TNLLLDDLKN APNKQPRCII VGSITGNTNT
+DSKVACMMTV RQMHQRFHDATGITFASLYPGCIAETGLFREHVPLFKTLFPPFQKYITKGYVSEEEAGRRLAAVISDPKLNKSGAYWSWSSTTGSFDNK
-LAGNVPPKAN LGDLSGLAAG VPAANPMMDG QEFNGAKAYK DSKVACMMTV RQMHQRFHDA
-TGITFASLYP GCIAETGLFR EHVPLFKTLF PPFQKYITKG YVSEEEAGRR LAAVISDPKL
-NKSGAYWSWS STTGSFDNK
 <br><br>
-DVR1
+DVR1<br>
-Mass: 37kDa
+Mass: 37kDa<br>
-Sequence:
+Sequence:<br>
-MAMAASRQAV RVAAAVDADY RKREPKDVRV LVVGPTGYIG KFVVKELVSR GYNVVAFARE
+MAMAASRQAVRVAAAVDADYRKREPKDVRVLVVGPTGYIGKFVVKELVSRGYNVVAFARENAGIKGKMGREDIVKEFHGAEVRFGSVLDPASLRDVAFKDPVDVVVSCLA
-NAGIKGKMGR EDIVKEFHGA EVRFGSVLDP ASLRDVAFKD PVDVVVSCLA SRTGGKKDSW
+SRTGGKKDSWLIDYTATKNSLDVARASGAKHFVLLSAICVQKPLLEFQKAKLQFESDLQAAGDITYSIVRPTAFFKSIAGQIDIVKKGNPYVMFGDGNLAACKPISEADLASF
-LIDYTATKNS LDVARASGAK HFVLLSAICV QKPLLEFQKA KLQFESDLQA AGDITYSIVR
+IADCVTEQNKVNKVLPIGGPSKAFTAKQQADLLFNITGLPPKYFPVPVALMDGMIGLFDSLAKLFPQLEDSAEFARIGKYYATESMLVYDEARGVYRKTKRLVTARTRWKTS
-PTAFFKSIAG QIDIVKKGNP YVMFGDGNLA ACKPISEADL ASFIADCVTE QNKVNKVLPI
+SLVQ
-GGPSKAFTAK QQADLLFNIT GLPPKYFPVP VALMDGMIGL FDSLAKLFPQ LEDSAEFARI
-GKYYATESML VYDEARGVYR KTKRLVTART RWKTSSLVQ
 <br><br>
-ChlP
+ChlP<br>
-Mass: 47kDa
+Mass: 47kDa<br>
-Sequence:
+Sequence: <br>
-MVIGGGPSGA CAAETLAKGG VETFLLERKL DNCKPCGGAI PLCMVEEFDL PMEIIDRRVT
+MVIGGGPSGACAAETLAKGGVETFLLERKLDNCKPCGGAIPLCMVEEFDLPMEIIDRRVTKMKMISPSNREVDVGKTLSETEWIGMCRREVFDDYLRNRAQKLGANIVNGL
-KMKMISPSNR EVDVGKTLSE TEWIGMCRRE VFDDYLRNRA QKLGANIVNG LFMRSEQQSA
+FMRSEQQSAEGPFTIHYNSYEDGSKMGKPATLEVDMIIGADGANSRIAKEIDAGEYDYAIAFQERIRIPDDKMKYYENLAEMYVGDDVSPDFYGWVFPKYDHVAVGTGTVVN
-EGPFTIHYNS YEDGSKMGKP ATLEVDMIIG ADGANSRIAK EIDAGEYDYA IAFQERIRIP
+KTAIKQYQQATRDRSKVKTEGGKIIRVEAHPIPEHPRPRRCKGRVALVGDAAGYVTKCSGEGIYFAAKSGRMAAEAIVEGSANGTKMCGEDAIRVYLDKWDRKYWTTYKVLD
-DDKMKYYENL AEMYVGDDVS PDFYGWVFPK YDHVAVGTGT VVNKTAIKQY QQATRDRSKV
+ILQKVFYRSNPAREAFVELCEDSYVQKMTFDSYLYKTVVPGNPLDDVKLLVRTVSSILRSNALRSVNSKSVNVSFGSKANEERVM
-KTEGGKIIRV EAHPIPEHPR PRRCKGRVAL VGDAAGYVTK CSGEGIYFAA KSGRMAAEAI
-VEGSANGTKM CGEDAIRVYL DKWDRKYWTT YKVLDILQKV FYRSNPAREA FVELCEDSYV
-QKMTFDSYLY KTVVPGNPLD DVKLLVRTVS SILRSNALRS VNSKSVNVSF GSKANEERVM
 AA
+<br><br>
+ChlG<br>
+Mass: 36.84kDa<br>
+Sequence: <br>
+MNQQATEEKSDTNSAARQMLGMKGAALETDIWKIRVQLTKPVTWIPLIWGVACGAAASGHYQWNNPTQIAQLLTCMMMSGPFLTGYTQTINDWYDREIDAINEPYRPIPS GRISERDVIVQIWVLLLGGIGLAYTLDQWAGHTTPVMLQLTIFGSFISYIYSAPPLKLKQSGWAGNYALGSSYIALPWWAGQALFGTLTLDVMALTIAYSLAGLGIAIVNDFKSI
+EGDRQ MGLQSLPVAFGVDTAKWICVSTIDVTQLGVAAYLAWGLHEELYGAVLLALILPQIYFQYKYFLPDPIANDVKYQASAQPFLVFGLLTAGLACGHHVNAVAA
+AASAAGAL
 ===References===
 [1]