Difference between revisions of "Part:BBa K1941001:Design"
(→References) |
m (→References) |
||
| Line 14: | Line 14: | ||
===References=== | ===References=== | ||
| − | - CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes, L.A. Gilbert et al., Cell 2013. | + | - CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes, L.A. Gilbert et al., Cell 2013. <br> |
- Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds, J.G. Zalatan et al., Cell 2015. | - Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds, J.G. Zalatan et al., Cell 2015. | ||
Latest revision as of 21:29, 18 October 2016
scTef1_PP7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The overall structure of the scRNA was taken form "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds" J.G.Zalatan et al., Cell 2015, but the 20 base pairs specificity sequence was replaced with a sequence targeting TEF1 from "CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes", L.A. Gilbert et al., Cell 2013.
Source
some source
References
- CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in Eukaryotes, L.A. Gilbert et al., Cell 2013.
- Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds, J.G. Zalatan et al., Cell 2015.