Difference between revisions of "Part:BBa K1923006"

Revision as of 20:18, 18 October 2016

NLS-FLAG-EGFP-dCas9-NLS encoding gene

This composite part is a fusion protein composed of a triple FLAG tag, two SV40 nuclear nuclear localization sequences, a GFP and a dCas9. Sv40 NLS enables the fusion protein to localize in the nuclear. GFP provides fluorescence signal convient for observation under microscope. Protein purification and expression detection is pretty with the help of FLAG tag. dCas9 can be used for CRISPR interference and DNA imaging. We can also utilize dCas9's RNA-binding ability with the help of sgRNA and PAMmer, a NGG-containing DNA oligo.

For more information, please refer to the Wiki page of Team Tsinghua.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1972
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4251
Illegal XhoI site found at 145
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1923006 short</partinfo>
-dCas9-GFP fusion perotein is a fusion enzyme.
+This composite part is a fusion protein composed of a triple FLAG tag, two SV40 nuclear nuclear localization sequences, a GFP and a dCas9. Sv40 NLS enables the fusion protein to localize in the nuclear. GFP provides fluorescence signal convient for observation under microscope. Protein purification and expression detection is pretty with the help of FLAG tag. dCas9 can be used for CRISPR interference and DNA imaging. We can also utilize  dCas9's RNA-binding ability with the help of sgRNA and PAMmer, a NGG-containing DNA oligo.
+For more information, please refer to the Wiki page of Team Tsinghua.
 <!-- Add more about the biology of this part here