Difference between revisions of "Part:BBa K1923002"

Revision as of 20:03, 18 October 2016

Scaffold msr-Scaffold msd-Reverse transcriptase encoding gene

The msr-msd sequence in the part is flanked by two inverted repeats. Once transcribed, the msr-msd RNA folds into a secondary structure guided by the base pairing of the inverted repeats and the msr-msd sequence. The RT enzyme recognizes this secondary structure and uses a conserved guanosine residue in the msr as a priming site to reverse transcripe the msd sequence and produce a hybrid RNA-ssDNA molecule called msDNA. The two BsaI sites enable researchers to design ssDNA sequence produced by this cassette, thus providing a programmable in-vivo ssDNA expression system ^[2].

For more information, please refer to the Wiki page of Team Tsinghua.

Reference:
[1] Farzadfard F, Lu T K. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations[J]. Science, 2014, 346(6211): 1256272.

Sequence and Features