Difference between revisions of "Part:BBa K2008008:Design"
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===Source=== | ===Source=== | ||
− | The pVeg promoter, sec secretion tag as well as the 125-bp <i>thrC</i> regions of homology <i>B. subtilis | + | The pVeg promoter, sec secretion tag as well as the 125-bp <i>thrC</i> regions of homology <i>B. subtilis</i> genome. TD1 is a transdermal tag first entered into the registry by the USTC_China iGEM team in 2013. |
===References=== | ===References=== |
Latest revision as of 19:41, 18 October 2016
pVeg->sec-TD1 modular secretion platform with ThrC homology
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 313
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Addition of the thrC homology regions are derived from B. subtilis 168 genomic DNA. Internal BamHI and XmaI restriction sites are present to allow for directional cloning of any desired insert. A secretory tag is present to allow for secretion of said insert, and a transdermal tag is present to allow the desired protein product to be secreted.
Source
The pVeg promoter, sec secretion tag as well as the 125-bp thrC regions of homology B. subtilis genome. TD1 is a transdermal tag first entered into the registry by the USTC_China iGEM team in 2013.