Difference between revisions of "Part:BBa K2008006:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Two internal EcoRI restriction sites and one internal SpeI restriction site to allow for this part to be BioBrick compatible. Codons were optimized for B. subtilis.
+
Two internal EcoRI restriction sites and one internal SpeI restriction site to allow for this part to be BioBrick compatible. Codons were optimized for <i>B. subtilis</i>.
  
  

Latest revision as of 19:29, 18 October 2016


Xylose->comK with removal of internal restriction sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 197
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 737


Design Notes

Two internal EcoRI restriction sites and one internal SpeI restriction site to allow for this part to be BioBrick compatible. Codons were optimized for B. subtilis.


Source

This part was amplified from the pAX01-comK plasmid constructed by Zhang and Zhang, 2010, and was previously characterized by the UofC_Calgary 2014 iGEM team.

References