Difference between revisions of "Part:BBa K2008003"

Latest revision as of 19:19, 18 October 2016

pVeg->sec-TD1-KSCI-BBI-GFP

This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. A B. subtilis secretory signal peptide sequence is included to allow for secretion of the peptide directly into surrounding media. A transdermal tag, TD1, (https://parts.igem.org/Part:BBa_K1074000) is fused to the N-terminus of BBI to allow for diffusion of the peptide across the skin.

This construct is fused to GFP (https://parts.igem.org/Part:BBa_E0040) for visual detection, and the entire coding sequence is under the control of the constitutive B. subtilis promoter pVeg (https://parts.igem.org/Part:BBa_K143012) and a strong B. subtilis RBS (https://parts.igem.org/Part:BBa_K780001).

Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 925

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2008003 short</partinfo>
-This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. A B. subtilis secretory signal peptide sequence is included to allow for secretion of the peptide directly into surrounding media. A transdermal tag (TD1, BBa_K1074000) is fused to the N-terminus of BBI to allow for diffusion of the peptide across the skin.
+This part contains an active nonamer from the Bowman Birk Protease Inhibitor (BBI) with the addition of lysine, serine, cysteine, and isoleucine (KSCI) tag on the N-terminal end and a phenylalanine (F) tag on the C-terminal end to increase BBI solubility. A <i>B. subtilis</i> secretory signal peptide sequence is included to allow for secretion of the peptide directly into surrounding media. A transdermal tag, TD1, (https://parts.igem.org/Part:BBa_K1074000) is fused to the N-terminus of BBI to allow for diffusion of the peptide across the skin.<br><br>
+This construct is fused to GFP (https://parts.igem.org/Part:BBa_E0040) for visual detection, and the entire coding sequence is under the control of the constitutive <i>B. subtilis</i> promoter pVeg (https://parts.igem.org/Part:BBa_K143012) and a strong <i>B. subtilis</i> RBS (https://parts.igem.org/Part:BBa_K780001).<br><br>
-This construct is fused to GFP (BBa_E0040) for visual detection, and the entire coding sequence is under the control of the constitutive B. subtilis promoter pVeg (BBa_K143012) and a strong B. subtilis RBS (BBa_K780001).
 Note: This part is a composite part, not a basic part, but it could not be added as composite at the time of entry.