Difference between revisions of "Part:BBa K1951004"

Revision as of 18:57, 18 October 2016

DesA producer under PBAD/araC promotor

This coding sequence is a producer of Lysine descarboxylase (Streptomyces coelicolor) which is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. There is cadeverin producing (1,5-diaminopentane), a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH. In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. This enzyme is also the first step in the production of Desferrioxamine B which is a siderophore.

The production of the protein has been controled by a PBAD promoter inductible with arabinose (0.01% arabinose added in the culture medium. (ref : Bba10500) The RBS used is the Bba B0034.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 25
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 388
Illegal NgoMIV site found at 1435
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1951004 short</partinfo>
-This coding sequence is a producer of Lysine descarboxylase (Streptomyces coelicolor) which is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. There is cadeverin producing (1,5-diaminopentane), a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.
+This coding sequence is a producer of Lysine descarboxylase (''Streptomyces coelicolor'') which is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. There is cadeverin producing (1,5-diaminopentane), a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.
-In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. This enzyme is also the first step in the production of desferrioxamine B which is a siderophore.
+In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor. This enzyme is also the first step in the production of Desferrioxamine B which is a siderophore.
 The production of the protein has been controled by a PBAD promoter inductible with arabinose (0.01% arabinose added in the culture medium. (ref : Bba10500)