Difference between revisions of "Part:BBa K1898400:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.  
+
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. The stop codon from CRYAB was also removed when designing the construct.  
  
 +
PCR primers were ordered to move CRYAB from IDT into an iGEM BioBrick:
 +
 +
forward: 5' ATATCTgCAgCggCC 3' (15)
 +
 +
reverse: 5' ATATCTgCAgCggCC 3' (15)
  
  
Line 14: Line 19:
 
===Source===
 
===Source===
  
−
the cDNA or cryab is ordered from OriGene.  
+
cryab is synthesized by IDT.
 +
 
 +
Primers were ordered from Tri-I Biotech.  
 +
 
  
 
===References===
 
===References===

Latest revision as of 18:48, 18 October 2016


CRYAB, crystallin alpha B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 26
    Illegal BamHI site found at 368
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 453
    Illegal SapI.rc site found at 313


Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. The stop codon from CRYAB was also removed when designing the construct.

PCR primers were ordered to move CRYAB from IDT into an iGEM BioBrick:

forward: 5' ATATCTgCAgCggCC 3' (15)

reverse: 5' ATATCTgCAgCggCC 3' (15)


Source

cryab is synthesized by IDT.

Primers were ordered from Tri-I Biotech.


References