Difference between revisions of "Part:BBa K2040119:Design"

(→‎Source)
(→‎Design Notes)
 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K2040122 short</partinfo>
+
<partinfo>BBa_K2040119 short</partinfo>
  
−
<partinfo>BBa_K2040122 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K2040119 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
aaa
+
Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Latest revision as of 18:44, 18 October 2016


mRFP1 + TtrpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1351
    Illegal AgeI site found at 555
    Illegal AgeI site found at 667
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1065


Design Notes

Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.

Source

mRFP1 orignated from Discosoma striata; TtrpC was isolated from pAN7-1.

References