Difference between revisions of "Part:BBa K1898300:Design"
Line 9: Line 9:
 
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the CRYAA DNA. Primers were designed to remove the stop codon and to move CRYAA into iGEM BioBrick. The primers are:
 
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the CRYAA DNA. Primers were designed to remove the stop codon and to move CRYAA into iGEM BioBrick. The primers are:
  
−
foward:  
+
foward: 5' ATATgAATTCgCggCCgCTTCTAgATggACgTgACCATCCAgCA 3' (44)
−
 
+
reverse: 5' ATATCTgCAgCggCCgCTACTAgTAggACgAgggAgCC 3' (38)
  
 
===Source===
 
===Source===
  
−
The cDNA of CRYAA is ordered from OriGene
+
The cDNA of CRYAA is ordered from OriGene.
−
 
+
Primers were ordered from Tri-I Biotech.
−
===References===
+

Revision as of 18:14, 18 October 2016


CRYAA, crystallin alpha A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the CRYAA DNA. Primers were designed to remove the stop codon and to move CRYAA into iGEM BioBrick. The primers are:

foward: 5' ATATgAATTCgCggCCgCTTCTAgATggACgTgACCATCCAgCA 3' (44) reverse: 5' ATATCTgCAgCggCCgCTACTAgTAggACgAgggAgCC 3' (38)

Source

The cDNA of CRYAA is ordered from OriGene. Primers were ordered from Tri-I Biotech.