Difference between revisions of "Part:BBa K2040100:Design"
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+ | Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) gene, complete cds.(DQ238489.1) http://www.ebi.ac.uk/ena/data/view/DQ238489 |
Revision as of 18:12, 18 October 2016
PMcl1, hemolymph inducible promoter from Metarhizium anisopliae
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1673
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 853
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1229
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1179
Illegal SapI site found at 1130
Design Notes
Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.
Source
It was isolated from the genomic DNA of Metarhizium anisopliae.
References
Metarhizium anisopliae collagen-like protein Mcl1 (Mcl1) gene, complete cds.(DQ238489.1) http://www.ebi.ac.uk/ena/data/view/DQ238489