Difference between revisions of "Part:BBa K2040100:Design"

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===Source===
 
===Source===
  
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It was isolated from the genomic DNA of our chassis organism ''Metarhizium anisopliae'' ARSEF549.
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It was isolated from the genomic DNA of ''Metarhizium anisopliae''.
  
 
===References===
 
===References===

Revision as of 17:55, 18 October 2016


PMcl1, hemolymph inducible promoter from Metarhizium anisopliae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1673
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 853
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1179
    Illegal SapI site found at 1130


Design Notes

Two illegal cutsites (XbaI and SpeI) were subsequently removed via site-directed mutagenesis.

Source

It was isolated from the genomic DNA of Metarhizium anisopliae.

References