Difference between revisions of "Part:BBa K1898300"
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==Gel Pictures==
 
==Gel Pictures==
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We digested CRYAA with Xbal and Pst1 enzymes. The expected band size is ~700 bp. As shown below, the bands with the expected size are boxed in red:  
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We digested CRYAA with Xbal and Pst1 enzymes. The expected band size is ~700 bp. In the gel picture shown below, lane 1 is 1kb ladder and bands with the expected size are boxed in red:  
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https://static.igem.org/mediawiki/parts/4/4f/Cryaa_pcr_gel_check.png  
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https://static.igem.org/mediawiki/parts/4/4f/Cryaa_pcr_gel_check.png
  
 
==Sequencing==
 
==Sequencing==

Revision as of 17:49, 18 October 2016


CRYAA, crystallin alpha A

This gene codes for CRYAA, or crystallin alpha A, a crystallin protein that is more restricted to the lens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]



Gel Pictures

We digested CRYAA with Xbal and Pst1 enzymes. The expected band size is ~700 bp. In the gel picture shown below, lane 1 is 1kb ladder and bands with the expected size are boxed in red:

Cryaa_pcr_gel_check.png

Sequencing

CRYAA DNA was sent to sequencing after it's moved into an iGEM backbone. The sequencing result confirms that CRYAA is right.

  • The four enzyme cutting sites are highlighted in red
  • CRYAA is highlighted in light blue

Cryaa.png