Difference between revisions of "Part:BBa K1898300"

 
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<partinfo>BBa_K1898300 short</partinfo>
 
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This gene codes for cryaa, or crystallin alpha A, a crystallin protein that is more restricted to the lens.  
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This gene codes for CRYAA, or crystallin alpha A, a crystallin protein that is more restricted to the lens.  
  
 
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===Usage and Biology===
 
===Usage and Biology===
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Alpha crystallin is the most abundant protein in the lens. Out of the two types of alpha crystallin proteins, alpha A is more restricted to the lens and has a 3:1 ratio to alpha B [1]. It has been found that crystallin alpha A has chaperone activity to maintain normal protein interaction [2].
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In our project, we made CRYAA because we wanted to monitor crystallin protein's response to oxidative stress and to test our prevention and treatment for cataracts.
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[1]Genes and mapped phenotypes. (n.d.). from https://www.ncbi.nlm.nih.gov/gene/1410
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[2]Usha, A. P. (n.d.). Crystallins in the eye: Function and pathology. from http://www.sciencedirect.com/science/article/pii/S1350946206000553
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<partinfo>BBa_K1898300 parameters</partinfo>
 
<partinfo>BBa_K1898300 parameters</partinfo>
 
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==Gel Pictures==
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We digested CRYAA with Xbal and Pst1 enzymes. The expected band size is ~700 bp. As shown below, the bands with the expected size are boxed in red:
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https://static.igem.org/mediawiki/parts/4/4f/Cryaa_pcr_gel_check.png
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==Sequencing==
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CRYAA DNA was sent to sequencing after it's moved into an iGEM backbone. The sequencing result confirms that CRYAA is right.
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*The four enzyme cutting sites are highlighted in red
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*CRYAA is highlighted in light blue
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https://static.igem.org/mediawiki/parts/c/cb/Cryaa.png

Revision as of 17:48, 18 October 2016


CRYAA, crystallin alpha A

This gene codes for CRYAA, or crystallin alpha A, a crystallin protein that is more restricted to the lens.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]



Gel Pictures

We digested CRYAA with Xbal and Pst1 enzymes. The expected band size is ~700 bp. As shown below, the bands with the expected size are boxed in red: Cryaa_pcr_gel_check.png

Sequencing

CRYAA DNA was sent to sequencing after it's moved into an iGEM backbone. The sequencing result confirms that CRYAA is right.

  • The four enzyme cutting sites are highlighted in red
  • CRYAA is highlighted in light blue

Cryaa.png