Difference between revisions of "Part:BBa K1675021:Experience"
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===Applications of BBa_K1675021=== | ===Applications of BBa_K1675021=== | ||
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| + | Austin_UTexas 2016 iGEM Team Characterization</h2> | ||
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| + | <p>Characterized by Austin UTexas in 2016 | ||
| + | <p>The above part served as the promoter in conjunction with the BioBrick <a href="http://partsregistry.org/Part:BBa_K592009">BBa_K592009</a> which served as the visual reporter for the design of a pH sensor for use in <i>E. coli</i>. A test was designed in which a plasmid containing the P-atp2 promoter with the blue chromoprotein was grown alongside an <i>E. coli</i> line that contained a plasmid with the blue chromoprotein and the promoter/RBS <a href="https://parts.igem.org/Part:BBa_K806002">BBa_K806002</a> (<a href="https://parts.igem.org/Part:BBa_K2097001">BBa_K209701</a>). We expected to see constant blue chromoprotein production in the control series (those that lacked P-atp2) and a gradual visual increase in blue chromoprotein as the pH was raised from 6 to 9 in the cells that contained the P-atp2 construct. | ||
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| + | <img src="https://static.igem.org/mediawiki/2016/4/46/T--Austin_UTexas--Patp2Results.png" alt="Patp2Results" style="width:80%;"> | ||
| + | <p><b>Figure 1</b> | ||
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| + | <p>As seen in figure 1, there is no clear gradual increase in blue chromoprotein in the P-atp2 trials suggesting that this promoter is not sensitive to basic pH changes. Spectrophotometer readings were unable to be taken due to the blue chromoprotein's absorption at 588nm interfering with the 600nm wavelength used to take cell counts, meaning quantitative data was not discernible between what was cell, and what was blue chromoprotein. | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 16:31, 18 October 2016
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Applications of BBa_K1675021
Austin_UTexas 2016 iGEM Team Characterization
Characterized by Austin UTexas in 2016
The above part served as the promoter in conjunction with the BioBrick BBa_K592009 which served as the visual reporter for the design of a pH sensor for use in E. coli. A test was designed in which a plasmid containing the P-atp2 promoter with the blue chromoprotein was grown alongside an E. coli line that contained a plasmid with the blue chromoprotein and the promoter/RBS BBa_K806002 (BBa_K209701). We expected to see constant blue chromoprotein production in the control series (those that lacked P-atp2) and a gradual visual increase in blue chromoprotein as the pH was raised from 6 to 9 in the cells that contained the P-atp2 construct.
Figure 1
As seen in figure 1, there is no clear gradual increase in blue chromoprotein in the P-atp2 trials suggesting that this promoter is not sensitive to basic pH changes. Spectrophotometer readings were unable to be taken due to the blue chromoprotein's absorption at 588nm interfering with the 600nm wavelength used to take cell counts, meaning quantitative data was not discernible between what was cell, and what was blue chromoprotein.
===User Reviews===