Difference between revisions of "Part:BBa K1899006"

Revision as of 15:34, 18 October 2016

J23101-B0032-lacl-B1006- phlFp-GFP

The construct aims at investigating any interference caused by lacl repressor on promoter phlFp.

Results

The fold change between construct A (pSB3K3-phlFp- BBa_E0240) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- phlFp -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between lacp andphlFp(BBa_K1899004).

Fig 1. Comparison on fluorescence expression levels of construct A (BBa_phlFp-E0240) and construct C (BBa_J23101-B0032-C0012-B1006- phlFp -E0240). Negative control represents BBa_E0240. Characterization was done using E. coli strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1209
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2007

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 ===Results===
+The fold change between construct A (pSB3K3-<i>phlFp</i>- BBa_E0240) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between <i>lacp</i> and<i>phlFp</i>(<bbpart>(BBa_K1899004</bbpart>).
-[[File:IT--Hong_Kong_HKUST--Phlfp_C.jpg/693px-T--Hong_Kong_HKUST--Phlfp_C.jpg.png|thumb|600px|center|<b>Fig 1. <b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240(C) and  pSB3K3-<i>phlFp</i> - BBa_E0240(A)</b>. Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]
+[[File:IGEM2016_HKUST_phlFK1899006.png|thumb|600px|center|<b>Fig 1. Comparison on fluorescence expression levels of construct A (BBa_<i>phlFp</i>-E0240) and construct C (BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240)</b>. Negative control represents BBa_E0240. Characterization was done using <i>E. coli</i> strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.]]
-<br><br>
-The fold change between <b>pSB3K3-<i>phlFp</i> - BBa_E0240(A) and negative control (pSB3K3-BBa_E0240)</b> is 13.2, which is same as that of <b>pSB3K3-BBa_J23101-B0032-C0012-B1006- <i>phlFp</i> -E0240(C)</b>.Mean and standard error mean(SEM) are calculated and represented by the height of the bar and the error bar respectively. Since their RFU are similar and almost the same, it proves that there is no cross-talk between <i>lacp</i> and<i>phlFp</i>(<bbpart>(BBa_K1899004</bbpart>).