Difference between revisions of "Part:BBa K1898550:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We made sure that there was no Ecor1, Xbal, Spel1, and Pst1 cutting sites in the DNA. The double stop codon at the end of BBa_E0040 was also removed.  
+
When we designed the GFP construct, we took out the double stop codon at the end of BBa_E0040.  
  
 +
The following primers were used to move the GFP construct from IDT into iGEM BioBrick:
  
 +
Forward: 5' ATATgAATTCgCggCCg 3' (17)
 +
 +
Reverse: 5' ATATCTgCAgCggCC 3' (15)
 +
  
 
===Source===
 
===Source===
  
 
This construct was designed and sent to IDT for oligo synthesis.  
 
This construct was designed and sent to IDT for oligo synthesis.  
−
 
+
Primers were synthesized by Tri-I BioTech
−
===References===
+

Latest revision as of 15:31, 18 October 2016


Strong promoter + Strong RBS + GFP + 10x Histidine tag + double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

When we designed the GFP construct, we took out the double stop codon at the end of BBa_E0040.

The following primers were used to move the GFP construct from IDT into iGEM BioBrick:

Forward: 5' ATATgAATTCgCggCCg 3' (17)

Reverse: 5' ATATCTgCAgCggCC 3' (15)


Source

This construct was designed and sent to IDT for oligo synthesis. Primers were synthesized by Tri-I BioTech