Difference between revisions of "Part:BBa K1899007"

Revision as of 15:23, 18 October 2016

phlFp-B0032-Lacl-B0032-TetR-GFP

This construct is designed to estimate the workload of E. coli and measure the fluorescence expression levels by the promoter when it is ligated to the tristable switch.

Results

Fig a ) Comparison of Technical Triplicate Results of pSB3K3-phlFp - BBa_B0032-C0012-B0032-C0040-E0240(E) and pSB3K3-phlFp- BBa_E0240(A)

The fold change between pSB3K3-phlFp-BBa_E0240(A) negative control (pSB3K3-BBa_E0240) and that of pSB3K3-phlFp-BBa_B0032-C0012-B0032-C0040-E0240(E) is 13.2 and 1.8 times respectively.

Owing to the increase of the number of coding regions, the workload of the cells is highly increased. The production rate of fluorescence should therefore be lower. It is acceptable to obtain a drop in the RFU level of pSB3K3-phlFp-BBa_B0032-C0012-B0032-C0040-E0240(E) comparing with pSB3K3-phlFp- BBa_E0240(A).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1240
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2629

@@ Line 10: / Line 10: @@
 https://static.igem.org/mediawiki/parts/thumb/9/95/Comparison_of_Construct_A_and_E.jpeg/693px-Comparison_of_Construct_A_and_E.jpeg
-Fig a ) <b>Comparison of Technical Triplicate Results of pSB3K3-<i>PhlFp</i> - BBa_B0032-C0012-B0032-C0040-E0240(E) and pSB3K3-<i>PhlFp</i> - BBa_E0240(A)</b>
+Fig a ) <b>Comparison of Technical Triplicate Results of pSB3K3-<i>phlFp</i> - BBa_B0032-C0012-B0032-C0040-E0240(E) and pSB3K3-<i>phlFp</i>- BBa_E0240(A)</b>
-The fold change between<b> pSB3K3-<i>PhlFp</i>-BBa_E0240(A)</b> negative control <b>(pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-<i>PhlFp </i>-BBa_B0032-C0012-B0032-C0040-E0240(E)</b> is 13.2 and 1.8 times respectively.
+The fold change between<b> pSB3K3-<i>phlFp</i>-BBa_E0240(A)</b> negative control <b>(pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-<i>phlFp</i>-BBa_B0032-C0012-B0032-C0040-E0240(E)</b> is 13.2 and 1.8 times respectively.
-Owing to the increase of the number of coding regions, the workload of the cells is highly increased. The production rate of fluorescence should therefore be lower. It is acceptable to obtain a drop in the RFU level of<b> pSB3K3-<i>PhlFp</i>-BBa_B0032-C0012-B0032-C0040-E0240(E)</b> comparing with <b>pSB3K3-<i>PhlFp</i>- BBa_E0240(A)</b>.
+Owing to the increase of the number of coding regions, the workload of the cells is highly increased. The production rate of fluorescence should therefore be lower. It is acceptable to obtain a drop in the RFU level of<b> pSB3K3-<i>phlFp</i>-BBa_B0032-C0012-B0032-C0040-E0240(E)</b> comparing with <b>pSB3K3-<i>phlFp</i>- BBa_E0240(A)</b>.