Difference between revisions of "Part:BBa K1899005"

Revision as of 14:45, 18 October 2016

J23101-B0032-TetR-B1006- phlFp -GFP

This part is constructed for investigating the effect of tetR over phlFp.

Results

Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- phlFp -E0240(D) and pSB3K3-phlFp- BBa_E0240(A)

The fold change between pSB3K3-phlFp - BBa_E0240(A), negative control (pSB3K3-BBa_E0240) and that of pSB3K3-BBa_J23101-B0032-C0040-B1006- phlFp -E0240(D) is around 13.2 times and 7.01 times respectively. Mean and standard error mean (SEM) are calculated and represented by the height of the bar and the error bar respectively.

This is due to the toxicity of BBa_C0040 (tetR). The toxicity reduces the growth rate of the E. coli containing this plasmid. The strong promoter BBa_J23101 makes this effect more significant when doing the characterisation. Though all the data were collected with OD within the mid-log range, there is still a variation between them, making a significant difference in the RFU. It is ungrounded to say tetR interferes the functionality of phlFp at this stage.
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1539

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1899005 short</partinfo>
-This part is constructed for investigating the effect of tetR over <i>PhlFp</i>.
+This part is constructed for investigating the effect of tetR over <i>phlFp</i>.
 <br>
 ===Results===
@@ Line 9: / Line 9: @@
 https://static.igem.org/mediawiki/parts/thumb/2/25/Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg/748px-Comparison_of_the_Strength_of_tetR_and_PhlFp.jpeg
-<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D) and  pSB3K3-<i>PhlFp</i>- BBa_E0240(A)</b>
+<b>Fig a) Comparison of Technical Triplicate Results of pSB3K3-BBa_J23101-B0032-C0040-B1006- <i>phlFp</i> -E0240(D) and  pSB3K3-<i>phlFp</i>- BBa_E0240(A)</b>
-The fold change between<b> pSB3K3-PhlFp - BBa_E0240(A)</b> and negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- PhlFp -E0240(D)</b> is around 13.2 times and 7.01 times respectively. Mean and standard error mean(SEM) are calculated and represented by the height of the bar and the error bar respectively.
+The fold change between<b> pSB3K3-<i>phlFp</i> - BBa_E0240(A)</b>, negative control<b> (pSB3K3-BBa_E0240)</b> and that of <b>pSB3K3-BBa_J23101-B0032-C0040-B1006- <i>phlFp</i> -E0240(D)</b> is around 13.2 times and 7.01 times respectively. Mean and standard error mean (SEM) are calculated and represented by the height of the bar and the error bar respectively.
 <br><br>
-This is due to the toxicity of <bbpart>BBa_C0040 </bbpart> (tetR). The toxicity reduces the growth rate of the<i> E. coli</i>  containing this plasmid. The strong promoter <bbpart>BBa_J23101</bbpart> makes this effect more significant when doing the characterisation. Though the data are collected with all OD within the mid-log range, there is still a distance between them, making a significant difference in the RFU. It is ungrounded to say tetR interferes the functionality of <i> PhlFp</i>  at this stage.
+This is due to the toxicity of <bbpart>BBa_C0040 </bbpart> (tetR). The toxicity reduces the growth rate of the <i> E. coli</i>  containing this plasmid. The strong promoter <bbpart>BBa_J23101</bbpart> makes this effect more significant when doing the characterisation. Though all the data were collected with OD within the mid-log range, there is still a variation between them, making a significant difference in the RFU. It is ungrounded to say tetR interferes the functionality of <i> phlFp</i>  at this stage.
 <br>
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