Difference between revisions of "Part:BBa K1979005"
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<img src="https://static.igem.org/mediawiki/parts/4/43/T--SDSZ_China--cbd-pbp_PCR.jpg" style="width:60%;"/><br/><br/> | <img src="https://static.igem.org/mediawiki/parts/4/43/T--SDSZ_China--cbd-pbp_PCR.jpg" style="width:60%;"/><br/><br/> | ||
<img src="https://static.igem.org/mediawiki/parts/6/60/T--SDSZ_China--cbd-pbp5_sds_.jpg" style="width:30%;"/"> | <img src="https://static.igem.org/mediawiki/parts/6/60/T--SDSZ_China--cbd-pbp5_sds_.jpg" style="width:30%;"/"> | ||
+ | <p class="text">Figure2. M: Marker; 1-3: deposit of E.coli after centrifugation<br/>The CBD-PBP5 protein (red box) is expressed, with a molecular weight of 53kDa. E.coli in well 3 is the blank comparison.</p> | ||
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Revision as of 13:50, 18 October 2016
CBD-PBP5
This part codes for CBD (cellulose binding domain)- PBP5 protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 135 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1322
Illegal BamHI site found at 168
Illegal XhoI site found at 1530 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 636
Illegal AgeI site found at 1238 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 184
Figure2. M: Marker; 1-3: deposit of E.coli after centrifugation
The CBD-PBP5 protein (red box) is expressed, with a molecular weight of 53kDa. E.coli in well 3 is the blank comparison.