Difference between revisions of "Part:BBa K1897024"

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<partinfo>BBa_K1897024 short</partinfo>
 
<partinfo>BBa_K1897024 short</partinfo>
  
The construct contains a shorter version of the promoter region for wild-type lldPRD operon ([[Part:BBa_K1897037]]) derived from  ([[Part:BBa_K822000]]) with a weaker RBS flanked by non-coding sequences ([[Part:BBa_K1897031]]), followed by a CDS of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]).
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The construct contains a shorter version of the promoter region for wild-type lldPRD operon ([[Part:BBa_K1897037]]) derived from  ([[Part:BBa_K822000]]) with a weaker RBS flanked by non-coding sequences ([[Part:BBa_K1897031]]), followed by a coding sequence of ALR ([[Part:BBa_K1172901]]) and a lambda t0 terminator ([[Part:BBa_K1897030]]).
  
  

Revision as of 13:37, 18 October 2016


p62-33-ALR-Terminator

The construct contains a shorter version of the promoter region for wild-type lldPRD operon (Part:BBa_K1897037) derived from (Part:BBa_K822000) with a weaker RBS flanked by non-coding sequences (Part:BBa_K1897031), followed by a coding sequence of ALR (Part:BBa_K1172901) and a lambda t0 terminator (Part:BBa_K1897030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 499
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 423
    Illegal BamHI site found at 1125
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 541
    Illegal AgeI site found at 841
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 298