Difference between revisions of "Part:BBa K1993035"
 
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In order to assemble our device easily since we had constructed plasmids by Gateway technology, we constructed a composite part transcripting IRES and Luciferase-T2A-dTomato-T2A-eGFP which is convenient for running Gateway BP and LR reactions.  
 
In order to assemble our device easily since we had constructed plasmids by Gateway technology, we constructed a composite part transcripting IRES and Luciferase-T2A-dTomato-T2A-eGFP which is convenient for running Gateway BP and LR reactions.  
 +
 
We introduced Internal ribosome entry site (IRES) (Details can be seen from [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016]) to ensure the translating initiation in an end-independent manner, as part of the greater process of protein synthesis.
 
We introduced Internal ribosome entry site (IRES) (Details can be seen from [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016]) to ensure the translating initiation in an end-independent manner, as part of the greater process of protein synthesis.
 +
 
Firefly(Photinus pyralis) Luciferase (Details can be seen from [https://parts.igem.org/Part:BBa_K1993018 BBa_K1993018]) is a kind of oxidative enzyme that produce bioluminescence. It is able to oxidize luciferin and produce detectable bioluminescence. It’s convenient to observe biological processes, especially allowing for observation of cells non-invasively. So we added such a gene to ensure observation in vivo.
 
Firefly(Photinus pyralis) Luciferase (Details can be seen from [https://parts.igem.org/Part:BBa_K1993018 BBa_K1993018]) is a kind of oxidative enzyme that produce bioluminescence. It is able to oxidize luciferin and produce detectable bioluminescence. It’s convenient to observe biological processes, especially allowing for observation of cells non-invasively. So we added such a gene to ensure observation in vivo.
 +
 
To expand the function of our composite part, we added two more genes: dTomato(Details can be seen from [https://parts.igem.org/Part:BBa_K1993020 BBa_K1993020])  used for observation in vitro conveniently and easily; hFTH(Details can be seen from [https://parts.igem.org/Part:BBa_K1993021 BBa_K1993021]), another protein that ccould be observed in vivo by MRI and applied to human body. What’s more, in order to make sure the expression of all three proteins, we added a T2A gene(Details can be seen from [https://parts.igem.org/Part:BBa_K1993019 BBa_K1993019]) between every two protein coding sequences. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.  
 
To expand the function of our composite part, we added two more genes: dTomato(Details can be seen from [https://parts.igem.org/Part:BBa_K1993020 BBa_K1993020])  used for observation in vitro conveniently and easily; hFTH(Details can be seen from [https://parts.igem.org/Part:BBa_K1993021 BBa_K1993021]), another protein that ccould be observed in vivo by MRI and applied to human body. What’s more, in order to make sure the expression of all three proteins, we added a T2A gene(Details can be seen from [https://parts.igem.org/Part:BBa_K1993019 BBa_K1993019]) between every two protein coding sequences. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.  
  

Latest revision as of 13:10, 18 October 2016


IRES-Luciferase-T2A-dtomato-T2A-hFTH

In order to assemble our device easily since we had constructed plasmids by Gateway technology, we constructed a composite part transcripting IRES and Luciferase-T2A-dTomato-T2A-eGFP which is convenient for running Gateway BP and LR reactions.

We introduced Internal ribosome entry site (IRES) (Details can be seen from BBa_K1993016) to ensure the translating initiation in an end-independent manner, as part of the greater process of protein synthesis.

Firefly(Photinus pyralis) Luciferase (Details can be seen from BBa_K1993018) is a kind of oxidative enzyme that produce bioluminescence. It is able to oxidize luciferin and produce detectable bioluminescence. It’s convenient to observe biological processes, especially allowing for observation of cells non-invasively. So we added such a gene to ensure observation in vivo.

To expand the function of our composite part, we added two more genes: dTomato(Details can be seen from BBa_K1993020) used for observation in vitro conveniently and easily; hFTH(Details can be seen from BBa_K1993021), another protein that ccould be observed in vivo by MRI and applied to human body. What’s more, in order to make sure the expression of all three proteins, we added a T2A gene(Details can be seen from BBa_K1993019) between every two protein coding sequences. By constructing such a composite part, we could run our Gateway BP and LR reactions more easily through adding the Gateway loci to both end of that composite part.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1396