Difference between revisions of "Part:BBa K1993007"
 
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<h2>Details</h2>
 
<h2>Details</h2>
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CXCL12 is one of most common chemokines in various inflammatory diseases, especially in inflammatory bowel diseases, which plays an important role in attracting T lymphocytes in high efficiency. Likewise, in order to give MSCs with similar chemotaxis, we engineered MSCs with coding sequence of CXCR4, the corresponding receptor of CXCL12.(Details could be seen on [https://parts.igem.org/Part:BBa_K1993003 BBa_K1993003])
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<li>CXCL12 is one of most common chemokines in various inflammatory diseases, especially in inflammatory bowel diseases, which plays an important role in attracting T lymphocytes in high efficiency. Likewise, in order to give MSCs with similar chemotaxis, we engineered MSCs with coding sequence of CXCR4, the corresponding receptor of CXCL12.(Details could be seen on [https://parts.igem.org/Part:BBa_K1993003 BBa_K1993003])
 
<br>
 
<br>
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Also, to testify the expression of CXCR4 and to label MSCs in vitro, we improved our design with eGFP, a modified form of GFP frequently used as a reporter of expression. (Details can be seen from [https://parts.igem.org/Part:BBa_K1993017 BBa_K1993017])
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<li>Also, to testify the expression of CXCR4 and to label MSCs in vitro, we improved our design with eGFP, a modified form of GFP frequently used as a reporter of expression. (Details can be seen from [https://parts.igem.org/Part:BBa_K1993017 BBa_K1993017])
 
<br>
 
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To make sure these two proteins express simultaneously, IRES is an indispensable part. As mentioned in the pages of basic parts, internal ribosome entry site (IRES) is an RNA element that allows for translation initiation in an end-independent manner, which ensures simultaneous expression of both CXCR4 and eGFP. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016])
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<li>To make sure these two proteins express simultaneously, IRES is an indispensable part. As mentioned in the pages of basic parts, internal ribosome entry site (IRES) is an RNA element that allows for translation initiation in an end-independent manner, which ensures simultaneous expression of both CXCR4 and eGFP. (Details could be seen on [https://parts.igem.org/Part:BBa_K1993016 BBa_K1993016])
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Latest revision as of 12:38, 18 October 2016


CXCR4-IRES-eGFP

Function

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we constructed a plasmid BBa_K1993007 under the control of EF-1α. 

Details

  • CXCL12 is one of most common chemokines in various inflammatory diseases, especially in inflammatory bowel diseases, which plays an important role in attracting T lymphocytes in high efficiency. Likewise, in order to give MSCs with similar chemotaxis, we engineered MSCs with coding sequence of CXCR4, the corresponding receptor of CXCL12.(Details could be seen on BBa_K1993003)
  • Also, to testify the expression of CXCR4 and to label MSCs in vitro, we improved our design with eGFP, a modified form of GFP frequently used as a reporter of expression. (Details can be seen from BBa_K1993017)
  • To make sure these two proteins express simultaneously, IRES is an indispensable part. As mentioned in the pages of basic parts, internal ribosome entry site (IRES) is an RNA element that allows for translation initiation in an end-independent manner, which ensures simultaneous expression of both CXCR4 and eGFP. (Details could be seen on BBa_K1993016)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 494
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]