Difference between revisions of "Part:BBa K1898250"
Line 3: Line 3:
 
<partinfo>BBa_K1898250 short</partinfo>
 
<partinfo>BBa_K1898250 short</partinfo>
  
−
BBa_K880005 is used to provide a strong promoter and strong rbs to maximize protein output. The construct codes for glutathione reductase (GSR) and 10x Histidine tag. GSR is a catalyst for the conversion of glutathione disulfide to glutathione and 10x Histidine tag is used for protein purification. BBa_B0015 consists of a double terminator to end transcription.  
+
BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for GSR (glutathione reductase) and a 10x Histidine-tag. GSR is a catalyst for the conversion of glutathione disulfide to glutathione and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.
 +
 
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:17, 18 October 2016


Strong promoter + Strong RBS + GSR + 10x Histidine tag + Double terminator

BBa_K880005 consists of a strong promoter and strong RBS and was used to maximize protein production. This construct codes for GSR (glutathione reductase) and a 10x Histidine-tag. GSR is a catalyst for the conversion of glutathione disulfide to glutathione and 10x Histidine-tag is used for protein purification. BBa__B0015 is a double terminator used to stop transcription.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 804
    Illegal BamHI site found at 1488
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1199
    Illegal SapI.rc site found at 1607


Gel Pictures

PCR was set up after the construct was cloned. The expected PCR band sizes are ~2.1kb, which is shown in lane __:

Sequencing

The DNA was sent to sequencing. The sequencing result is correct and shown below: