Difference between revisions of "Part:BBa K2088007"
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PbaB is a [2Fe-2S]-type ferredoxin, found in ''Sphingobium wenxiniae'' JZ-1.It can express in E.coli（DH5ɑ or BL21) in plasmid pPBA. | PbaB is a [2Fe-2S]-type ferredoxin, found in ''Sphingobium wenxiniae'' JZ-1.It can express in E.coli（DH5ɑ or BL21) in plasmid pPBA. | ||
This part encodes a kind of protein named '''2Fe-2S ferredoxin''', a 2Fe-2S iron-sulfur cluster binding domain. Iron-sulfur proteins play an important role in electron transfer processes and in various enzymatic reactions. | This part encodes a kind of protein named '''2Fe-2S ferredoxin''', a 2Fe-2S iron-sulfur cluster binding domain. Iron-sulfur proteins play an important role in electron transfer processes and in various enzymatic reactions. | ||
| − | Mostly, pbaB implements its function with pbaA1A2. PbaA1A2B are the oxygenase and ferredoxin components of the angular dioxygenase (3-phenoxybenzoate 1’,2’-dioxygenase) responsible for the angular dioxygenation at the 1’ and 2’ positions on the benzene moiety of 3-phenoxybenzoate and the ferredoxin PbaB is indispensable for the angular dioxygenase. | + | |
| + | Mostly, pbaB implements its function with pbaA1A2([https://parts.igem.org/Part:BBa_K2088003 BBa_K2088003]). PbaA1A2B are the oxygenase and ferredoxin components of the angular dioxygenase (3-phenoxybenzoate 1’,2’-dioxygenase) responsible for the angular dioxygenation at the 1’ and 2’ positions on the benzene moiety of 3-phenoxybenzoate and the ferredoxin PbaB is indispensable for the angular dioxygenase. | ||
Latest revision as of 10:02, 18 October 2016
pbaB: a 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain.
PbaB is a [2Fe-2S]-type ferredoxin, found in Sphingobium wenxiniae JZ-1.It can express in E.coli（DH5ɑ or BL21) in plasmid pPBA. This part encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. Iron-sulfur proteins play an important role in electron transfer processes and in various enzymatic reactions.
Mostly, pbaB implements its function with pbaA1A2(BBa_K2088003). PbaA1A2B are the oxygenase and ferredoxin components of the angular dioxygenase (3-phenoxybenzoate 1’,2’-dioxygenase) responsible for the angular dioxygenation at the 1’ and 2’ positions on the benzene moiety of 3-phenoxybenzoate and the ferredoxin PbaB is indispensable for the angular dioxygenase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 228
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]