Difference between revisions of "Part:BBa K2016000"
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<partinfo>BBa_K2016000 short</partinfo> | <partinfo>BBa_K2016000 short</partinfo> | ||
| − | + | BBa_K2016000 is a derivative of Bba_J23100, a promoter from a family of constitutive <i>E. coli</i> promoters isolated from a small combinatorial library. This family was submitted to the registry by Berkeley 2006. Sheffield 2016 has improved Bba_J23100 by adding an RBS downstream of the promoter to make it suitable for direct cloning. | |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
| − | <partinfo>BBa_K2016000 | + | <partinfo>BBa_K2016000 is 91bp long, first 35 nucleotides make up a functional strong constitutive promoter for expression in <i>E. coli</i>, followed by a 50 nucleotide spacer (5' UTR) and ending with a 6 nucleotide ribosomal binding site (AGGAGG). </partinfo> |
Revision as of 08:44, 18 October 2016
Strong constitutive E. coli promoter with included RBS - ready for cloning
BBa_K2016000 is a derivative of Bba_J23100, a promoter from a family of constitutive E. coli promoters isolated from a small combinatorial library. This family was submitted to the registry by Berkeley 2006. Sheffield 2016 has improved Bba_J23100 by adding an RBS downstream of the promoter to make it suitable for direct cloning.
Usage and Biology
Sheffield 2016 has used this promoter in order to design a biological device that responds to intracellular iron levels in bacteria. Bba_J23106 was also used in order to observe a difference between having a system under the control of a medium and a strong promoter.
Sequence and Features BBa_K2016000 is 91bp long, first 35 nucleotides make up a functional strong constitutive promoter for expression in E. coli, followed by a 50 nucleotide spacer (5' UTR) and ending with a 6 nucleotide ribosomal binding site (AGGAGG). Not understood