Difference between revisions of "Part:BBa K2074022"
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our part is cry10Aa which have toxicity to the larve of mosquit link with 2A peptide.We transferred the gene cry10Aa+2A into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein . | our part is cry10Aa which have toxicity to the larve of mosquit link with 2A peptide.We transferred the gene cry10Aa+2A into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein . | ||
Revision as of 08:33, 18 October 2016
Cry10Aa(Codon optimization)-Extended FMDV
our part is cry10Aa which have toxicity to the larve of mosquit link with 2A peptide.We transferred the gene cry10Aa+2A into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 368
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 32
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 181
Illegal NgoMIV site found at 223
Illegal NgoMIV site found at 280
Illegal NgoMIV site found at 784
Illegal NgoMIV site found at 1460
Illegal NgoMIV site found at 2116 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 202
Illegal SapI.rc site found at 924