Difference between revisions of "Part:BBa K2074014"

Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2074014 short</partinfo>
 
<partinfo>BBa_K2074014 short</partinfo>
 
+
2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as Figure7. According to the Thomas’s research, 2A peptide system has proved the high-efficiency of co-expression and cleavage in Chlamydomonas
 
LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP
 
LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP
  

Revision as of 07:31, 18 October 2016


Extended FMDV 2A for Chlamydomonas reinhardtii 2A peptides system was firstly discovered in Foot-and-mouth disease virus (FMDV). 2A peptide is a linker which used to separate functional proteins between upstream and downstream, and the length is about 18-22 amino acids long. ‘Ribosomal skip’ occurs during translation that result in production of independent peptides or proteins from one polycistronic. After cleavage, the 2A peptide remains fused to the C-terminus of the upstream protein when the proline is linked to the N-terminus of the downstream as Figure7. According to the Thomas’s research, 2A peptide system has proved the high-efficiency of co-expression and cleavage in Chlamydomonas LLAIHPTEARHKQKIVAPVKQTLNFDLLKLAGDVESNPGP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 91
  • 1000
    COMPATIBLE WITH RFC[1000]