Difference between revisions of "Part:BBa K2074003"
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This devise is consisted of a HSP70A-RBCS2 promoter, Cry4Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
 
This devise is consisted of a HSP70A-RBCS2 promoter, Cry4Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii.
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[[File:2016FAFUCHINADevice1.png|420px|thumb|left|'''Fig.1''' Schematic structure of CecropinXJ with promotor J23106
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2074003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2074003 SequenceAndFeatures</partinfo>
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[[File:2016FAFUCHINADevice1.png|420px|thumb|left|'''Fig.1''' Schematic structure of CecropinXJ with promotor J23106
 
  
 
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Revision as of 07:08, 18 October 2016


Cry4Aa+2A+Cyt1 expressed in C. reinhardtii

This devise is consisted of a HSP70A-RBCS2 promoter, Cry4Aa toxin, extended FMDV 2A peptides, a Cyt1 toxin and also a terminator. The HSP70A-RBCS2 promoter is a heat-induced promoter. Both Cry and Cyt are pore-froming toxins from Bacillus thuringiensis produced as parasporal crystal components. The synergism between the two toxins was observed against dipteran insects such as mosquitoes. It has been proved that the synergism can contribute to the suppression of resistance. The FMDV 2A peptides serves as a linker between a upstream protein and a downstream protein in order to co-express them in a single vector. The device we designed are all codon-optimized so that we can safely express the toxins via Chlamydomonas reinhardtii. [[File:2016FAFUCHINADevice1.png|420px|thumb|left|Fig.1 Schematic structure of CecropinXJ with promotor J23106

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1641
    Illegal BglII site found at 4452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2033
    Illegal NgoMIV site found at 4286
    Illegal NgoMIV site found at 4586
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 686
    Illegal BsaI.rc site found at 839
    Illegal BsaI.rc site found at 2726
    Illegal BsaI.rc site found at 3266
    Illegal BsaI.rc site found at 3575
    Illegal BsaI.rc site found at 4142
    Illegal SapI.rc site found at 1843
    Illegal SapI.rc site found at 1885