Difference between revisions of "Part:BBa K2097002"

Line 11: Line 11:
 
<p><a href="https://parts.igem.org/Part:BBa_K1033916"> BBa_K1033916</a> was created by Uppasala 2013. It comes from the coral Anemonia majano. <a href="https://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> was created by Freiburg 2011.
 
<p><a href="https://parts.igem.org/Part:BBa_K1033916"> BBa_K1033916</a> was created by Uppasala 2013. It comes from the coral Anemonia majano. <a href="https://parts.igem.org/Part:BBa_K608006"> BBa_K608006</a> was created by Freiburg 2011.
 
</html>
 
</html>
 
+
<div class="column full_size">
 
[[File:YGCPtube.png|thumb|left|275px|Figure 1. amajLime expressed in <i>E. coli</i> in liquid LB]]
 
[[File:YGCPtube.png|thumb|left|275px|Figure 1. amajLime expressed in <i>E. coli</i> in liquid LB]]
 
[[File:T--Austin_UTexas--YGCPspin.png|thumb|center|300px| Figure 2. amajLime expressed in <i>E. coli</i> after being spun down in a microcentrifuge]]
 
[[File:T--Austin_UTexas--YGCPspin.png|thumb|center|300px| Figure 2. amajLime expressed in <i>E. coli</i> after being spun down in a microcentrifuge]]
 
+
</div>
 
===Sequence and Features===
 
===Sequence and Features===
  

Revision as of 05:01, 18 October 2016


Yellow-green chromoprotein with medium promoter and RBS

This is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein) and BBa_K608006 (a medium promoter and RBS). It is made up of a medium promoter, BBa_J23110, and a medium RBS, BBa_B0032.

Usage and Biology

BBa_K1033916 was created by Uppasala 2013. It comes from the coral Anemonia majano. BBa_K608006 was created by Freiburg 2011.

Figure 1. amajLime expressed in E. coli in liquid LB
Figure 2. amajLime expressed in E. coli after being spun down in a microcentrifuge

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]