Difference between revisions of "Part:BBa K1919303"

 
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LivH is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. LivH encodes a very hydrophobic protein with 7 or 9 predicted transmembrane regions.  
 
LivH is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. LivH encodes a very hydrophobic protein with 7 or 9 predicted transmembrane regions.  
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This device constructed in our team contains a promoter J23100, a RBS, livH coding region and a terminator. The device can be transferred into E.coli and overexpress livH directly. The expression of the livH RNA in the part has been studied via qPCR.The quantified result suggests that compared with negtive control of E.coli (not cantain the plasmid), livH has been highly expressed in E.coli BL21 and E.coli DH5a.
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This device constructed in our team contains a promoter J23100, a RBS B0030 , livH coding region and a terminator B0010. The device can be transferred into E.coli and overexpresses livH directly. The expression of the livH RNA in the cell has been studied via qPCR. The quantified result suggests that compared with negative control E.coli (not cantain any plasmid), livH has been highly expressed in E.coli BL21 and E.coli DH5a.
  
 
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Revision as of 04:44, 18 October 2016


livH

LivH is an integral membrane component of the LIV-I (LivJHMGF) and LS (LivKHMGF) branched chain amino acid and phenylalanine ABC transport system in E. coli K-12. The LivFGHM proteins interact with either of two periplasmic binding proteins, LivJ or LivK, and catalyse the uptake of the branched chain amino acids, L-leucine, L-isoleucine and L-valine and the nonpolar, aromatic amino acid, phenylalanine. LivH encodes a very hydrophobic protein with 7 or 9 predicted transmembrane regions. This device constructed in our team contains a promoter J23100, a RBS B0030 , livH coding region and a terminator B0010. The device can be transferred into E.coli and overexpresses livH directly. The expression of the livH RNA in the cell has been studied via qPCR. The quantified result suggests that compared with negative control E.coli (not cantain any plasmid), livH has been highly expressed in E.coli BL21 and E.coli DH5a.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 631
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 953
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 910